Effect of linear alkylbenzene sulfonate (LAS) and atrazine on marine microalgae   总被引：1，自引：0，他引：1  

Debelius B  Forja JM  Del Valls A  Lubián LM 《Marine pollution bulletin》2008,57(6-12):559-568

Five marine microalgae (Tetraselmis chuii, Rhodomonas salina, Chaetoceros sp., Isochrysis galbana (T-iso) and Nannochloropsis gaditana), in the same biovolume quantity, were exposed to 72h growth-inhibition tests with atrazine and LAS. In all cases, the inhibition effect of atrazine was higher than that of LAS up to two orders of magnitude higher in the case of T. chuii. In a second part of the study, initial cellular densities for each microalga strain and fixed organic compound concentration were varied, and results show density has a clear influence in growth inhibition tests. Finally, the sum of all data obtained in the study was expressed in terms of "toxic cellular quota" (mass of chemical substance per cell). The result was a sigmoid curve with a good fit, including the two main factors in toxicity tests, initial cellular density and contaminant concentration. This toxic cellular quota exhibits a general tendency to increase with cell volume/size.  相似文献   

The effect of nitric oxide on the growth of marine phytoplankton   总被引：13，自引：0，他引：13  

ZHANG Zhengbin  LIN Cai  LIU Chunying  SUN Mingyi  DING HaibingThe Institute of Marine Chemistry  Ocean University of China  Qingdao  P.R.ChinaThe University of Georgia  Department of Marine Sciences  Athen GA   《中国海洋大学学报(英文版)》2003,2(2):185-188

The incubation experiments of Skeletonema costatum, Dicrateria zhanjiangensis nov. sp., and Platymonas sub-cordiformis, and those of Emiliania huxleyi were carried out in the Marine Physical Chemistry Laboratory in Ocean University of China and in the Marine Organic Geochemistry Laboratory in the University of Georgia respectively. Nitric oxide was added into the media when these marine microalgae were growing. We found the growth of these four microalgae were promoted or inhibited when nitric oxide of different concentrations was added one or two times each day during the cultivation process. The results are consistent with the influence of nitric oxide on the growth of high plants. The results show that nitric oxide may be a new factor of regulation and control for the phytoplankton growth in seawater.  相似文献   

Competition between pelagic and benthic microalgae for phosphorus and light   总被引：2，自引：0，他引：2  

Sabine Flöder  Anja Combüchen  Annika Pasternak  Helmut Hillebrand 《Aquatic Sciences - Research Across Boundaries》2006,68(4):425-433

  相似文献   

Resistant biomacromolecules in marine microalgae of the classes Eustigmatophyceae and Chlorophyceae: Geochemical implications     

Franois Gelin  Ilco Boogers  Anna A. M. Noordeloos  Jaap S. Sinninghe Damste  Roel Riegman  Jan W. De Leeuw 《Organic Geochemistry》1997,26(11-12)

Non-hydrolysable macromolecular constituents (i.e. algaenans) were isolated from two out of seven marine microalgae investigated. Nannochloropsis salina and Nannochloropsis sp. from the class of Eustigmatophyceae produce highly aliphatic algaenans. Flash pyrolysis and chemical degradations with HI and RuO₄ allowed for the identification of their chemical structure, which is mainly composed of polyether-linked long-chain (up to C₃₆) n-alkyl units. The building blocks of this polymer were also recognized in lipid fractions. The green microalgae (Chlorophyceae) Chlorella spaerckii, Chlorococcum sp. and Nannochloris sp. were earlier thought to biosynthesize algaenans comprising aliphatic and/or aromatic moieties. However, a new isolation method utilizing trifluoroacetic acid (TFA) prior to the other hydrolyses revealed that the macromolecular material isolated from these three chlorophytes was either hydrolysable with TFA or artefacts from the former method. Similar to algaenans from fresh water green microalgae, the aliphatic eustigmatophyte algaenans are likely to be selectively preserved in depositional environments and might ultimately serve as source rock organic matter of marine crude oils. Furthermore, they may play an important role in the cycling of carbon.  相似文献   

甾醇在海洋微藻中的分布研究   总被引：1，自引：0，他引：1       下载免费PDF全文

徐继林  严小军  周成旭  马斌 《海洋学报》2007,29(6):93-100

对29种(株)海洋微藻进行不充气培养,用Bligh-Dyer法提取总脂,皂化后氯仿和正己烷(体积比为1:4)提取甾醇,BSTFA衍生后用岛津QP2010气相色谱-质谱分析仪进行GC/MS分析.从29种海洋微藻中共检测到47种甾类化合物,鉴定出36种甾醇和一种甾酮.其中,4位无甲基Δ5甾醇在所有的微藻中均有分布,一些高等生物中常见的甾醇如胆甾醇、菜子甾醇、菜油甾醇、豆甾醇、谷甾醇和褐藻中常见的岩藻甾醇等,在微藻中均有广泛的分布.相对而言,4位无甲基5位饱和甾(烷)醇、4-甲基甾(烷)醇和双羟基甾醇的分布则相对较少,其中许多甾醇如甲藻甾醇、巴夫甾醇、C_27:2(Δ5,22/24Me)和C_28:3(Δ5,7,22/24Me)等,只在一种藻或同一纲的微藻中被检测到,可以作为对应微藻或对应纲微藻的特征甾醇.认为海洋微藻甾醇的研究可以为海洋微藻化学分类学及饵料营养学研究提供重要参考依据.  相似文献   

温度、光照、pH值对后棘藻生长及脂肪酸含量的影响   总被引：3，自引：0，他引：3  

徐年军  张学成 《中国海洋大学学报(自然科学版)》2001,31(4):541-547

报道了环境因子对富含 EPA (2 0∶ 5ω3)的海洋微藻后棘藻 (Ellipsoidion sp.) 70 - 0 1的生长速度、总脂及脂肪酸含量的影响。结果表明 ,后棘藻具有较快的生长速度和较高的脂肪酸含量 ,总脂含量为 31～ 36 % ,主要脂肪酸为 14∶ 0、16∶ 0、16∶ 1、18∶ 1ω9、18∶ 1ω7、18∶ 2 ω6、2 0∶ 4 ω6、2 0∶ 5ω3。生长的温度范围为 15～ 30℃ ,2 5℃时生长速度最快。温度对总脂含量影响很小 ,但对EPA和 PUFA含量影响较大。在 2 5℃时有最大的 EPA和 PUFA含量。适宜光强为 10 8.75μmolm-2 s-1～ 2 4 4 .15μmolm-2 s-1,在 145.54μmolm-2 s-1时 EPA产率较大。在起始 p H6 .5～ 9范围内 ,p H8.5时有最大的生长速率和总脂含量 ,而 EPA和 PUFA在起始 p H7.5时最大 ,分别占脂肪酸的 18.77%和 2 3.38%。实验条件下后棘藻 EPA产率最大的条件为温度 2 5℃ ,光强 145.54μmolm-2 s-1,p H为 7.5～ 8.5。  相似文献   

Dynamics of the Polychaetes Desdemona ornata and Perinereis cultrifera and Their Resources of Food in a Mediterranean Mudflat     

Francesca Rossi 《Marine Ecology》2002,23(4):285-296

Abstract. Availability of food in the sediment can play an important role in determining the dynamics of deposit feeders. Generally the abundance of deposit feeders during winter and spring is related to an increase in the nutritive value of the sediment due to the concurrent microalgal peaks. This work investigated, in 1998, the seasonal variability patterns in the nutritive value of the sediment and in the abundance of two polychaetes, Desdemona ornata (Sabellidae: Fabriicinae) and Perinereis cultrifera (Nereididae), that probably feed on benthic microalgae. Previously, in 1997, these two species were abundant in winter and spring. During that winter, the amount of chlorophyll a in the sediment was quite variable due to grazing activities. Three manipulative field experiments were carried out in winter and spring (January, March and April 1998) to test the hypothesis that increasing the amount of chlorophyll a increases the nutritional value of the sediment and the abundance of Perinereis cultrifera and Desdemona ornata . The results indicated that the patterns of variability for these species and for chlorophyll a were similar to those observed in 1997. In plots where microalgal growth was stimulated experimentally, Perinereis cultrifera increased in January and Desdemona ornata increased in January and March. The role of food in regulating the abundances of Perinereis cultrifera and Desdemona ornata is discussed.  相似文献   

Effect of UV-B radiation on the feeding behavior of the rotifer Brachionus plicatilis   总被引：3，自引：0，他引：3  

FENG Lei  LI Xin  WANG Jinhe  HAN Honglei  TANG Xuexi  CHEN Xiguang 《海洋学报(英文版)》2007,26(4):82-92

Effect of UV-B radiation on the feeding behaviour of marine zooplankton is important to assessing the health harm of marine ecosystem due to the gradually enhanced UV-B radiation in air. However, there are a few studies on this topic. The feeding behavior of the rotifer, Brachionus plicatilis, under the treatment of UV-B radiation on five species of microalgae, i.e., Chlorella sp., Tetraselmis chuii, Isochrysis galbana Park 8701, Chaetoceros muelleri Lermumerman, and Nitzschia clostertum, was studied. The results showed that the filtering and feeding rates of the rotifer decreased significantly with the dose increase of UV-B radiation when fed with five species of microalgae respectively (P<0.05) which indicates UV-B radiation inhibits the feeding activities of the rotifer on microalage. The mixed culture experiments shows the rotifer preferred to feed Chlorella sp., then C. muelleri,I. galbana, N. clostertum and T. chuii in turn if without UV-B radiation. Under the highest dose of UV-B radiation treatment (2.70 kJ/m2), the rotifer preferred to feed C. muelleri, then Chlorella sp., N. clostertum, I. galbana and T. chuii in turn. Chlorella sp., I. galbana and C. muelleri became the more favorite foods of the rotifer while T. chuii and N. clostertum became less favorite foods. The change of feeding rate and feeding selectivity of zooplankton driven by the enhanced UV-B radiation will lead to the change in the structure of phytoplankton community.  相似文献   

Effect of Fe3+ on the growth and lipid content of Isochrysis galbana     

刘志媛  王广策 《中国海洋湖沼学报》2014,32(1):47-53

Inducing lipid accumulation in microalgae cells without suppressing cell growth is vital to the economical production of biodiesel from microalgae. In two experiments, we demonstrate that the cell concentration and lipid content of marine microalgae Isochrysis galbana depend upon the iron concentration in the growth media. In Experiment I, adding chelated FeCl 3 to the medium at the late exponential growth phase prolonged this phase and increased the lipid content in I. galbana cells. The fi nal cell density and lipid content of I. galbana supplemented with chelated FeCl 3 was approximately 2 and 1.65 times higher than that of non-supplemented cultures, respectively. In Experiment II, I. galbana cells in the late exponential phase were collected and re-inoculated into new media containing Fe 3+ at various concentrations. The fi nal cell concentration and lipid content were maximized at the highest iron concentration(38% biomass by dry weight at 1.2×10-5 mol/L Fe 3+). In this study, intracellular neutral lipid storage was evaluated by fl uorescent spectrophotometry using fl uorochrome Nile red, and the measurement conditions were optimized.  相似文献   

多氯联苯在两种海洋微藻中毒性效应及富集效应的研究     

吴越  陈星星  潘齐存  郑伊诺  胡园  王瑶华  陆荣茂  黄振华 《海洋科学》2017,41(3):61-67

通过分析2,2',3,4,4',5'-六氯联苯(PCB_(138))对牟氏角毛藻(Chaetoceros muelleri)和亚心形扁藻(Platymonas subcordiformis)生长的影响,以及PCB_(138)对两种微藻超氧化物歧化酶(Superoxide dismutase,SOD)活性的影响,研究多氯联苯对海洋微藻的毒性效应,并采用气相色谱法研究了两种微藻对PCB_(138)的生物富集效应。结果表明,PCB_(138)对牟氏角毛藻和亚心形扁藻均有明显的毒性效应作用,对2种藻类的96 h半抑制质量浓度(96hEC_(50))分别为0.057 mg/和0.354 mg/L,与亚心形扁藻相比,牟氏角毛藻对PCB_(138)更敏感,耐受性更差;低浓度的PCB_(138)对两种微藻SOD酶活性有诱导作用,但当PCB_(138)浓度过高时,SOD酶活性下降;两种微藻对PCB_(138)均有明显的富集作用,随着培养时间的延长,牟氏角毛藻中富集的PCB_(138)含量呈上升趋势,而亚心形扁藻中PCB_(138)含量呈现先上升后下降趋势。  相似文献